magic pcr preps dna purification system Search Results


nebnext dna sample prep kit  (New England Biolabs)
97
New England Biolabs nebnext dna sample prep kit
Nebnext Dna Sample Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
nebnext dna sample prep kit - by Bioz Stars, 2026-10
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dna isolation kit  (Quanta Biosciences)
96
Quanta Biosciences dna isolation kit
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Dna Isolation Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/Extracta+DNA+Prep+for+PCR/bio_rxiv__2025__09__24__678094-36-14-17
Average 96 stars, based on 1 article reviews
dna isolation kit - by Bioz Stars, 2026-10
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wizard pcr preps dna purification system  (Promega)
90
Promega wizard pcr preps dna purification system
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Wizard Pcr Preps Dna Purification System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/wizard+genomic+dna+purification+kit/pm15377602-26-20-26
Average 90 stars, based on 1 article reviews
wizard pcr preps dna purification system - by Bioz Stars, 2026-10
90/100 stars
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wizard pcr preps dna purification kit  (Promega)
90
Promega wizard pcr preps dna purification kit
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Wizard Pcr Preps Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/wizard+pcr+preps+dna+purification+kit/pmc01570497-144-7-13
Average 90 stars, based on 1 article reviews
wizard pcr preps dna purification kit - by Bioz Stars, 2026-10
90/100 stars
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next ultra ii dna library prep kit  (New England Biolabs)
99
New England Biolabs next ultra ii dna library prep kit
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Next Ultra Ii Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/NEBNext+Ultra+II+DNA+Library+Prep+Kit+for+Illumina/pm35991434-92-13-20
Average 99 stars, based on 1 article reviews
next ultra ii dna library prep kit - by Bioz Stars, 2026-10
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xt v2 dna library prep kit  (Nextera AS)
90
Nextera AS xt v2 dna library prep kit
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Xt V2 Dna Library Prep Kit, supplied by Nextera AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/dna+library+preparation+kit/pmc09593052-141-13-12
Average 90 stars, based on 1 article reviews
xt v2 dna library prep kit - by Bioz Stars, 2026-10
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dna affinity spin columns wizard pcr preps  (Promega)
90
Promega dna affinity spin columns wizard pcr preps
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Dna Affinity Spin Columns Wizard Pcr Preps, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/spin+columns+wizard+pcr+prep/pm10469313-54-10-17
Average 90 stars, based on 1 article reviews
dna affinity spin columns wizard pcr preps - by Bioz Stars, 2026-10
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neb next ultra ii fs dna pcr  (New England Biolabs)
97
New England Biolabs neb next ultra ii fs dna pcr
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Neb Next Ultra Ii Fs Dna Pcr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/NEBNext+Ultra+II+FS+DNA+PCR-free+Library+Prep+Kit+for+Illumina/10__1016_slash_j__aqrep__2024__102576-64-1-1
Average 97 stars, based on 1 article reviews
neb next ultra ii fs dna pcr - by Bioz Stars, 2026-10
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nebnext ultra ii directional kit neb cat  (New England Biolabs)
99
New England Biolabs nebnext ultra ii directional kit neb cat
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Nebnext Ultra Ii Directional Kit Neb Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/NEBNext+Ultra+II+Directional+RNA+Library+Prep+Kit+for+Illumina/pm40675154-266-222-227
Average 99 stars, based on 1 article reviews
nebnext ultra ii directional kit neb cat - by Bioz Stars, 2026-10
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wizard pcr prep kit  (Promega)
90
Promega wizard pcr prep kit
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Wizard Pcr Prep Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/reverse+transcription+kit/10__1128_slash_aem__71__4__1816___1821__2005-66-14-18
Average 90 stars, based on 1 article reviews
wizard pcr prep kit - by Bioz Stars, 2026-10
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nebnext sample purification beads  (New England Biolabs)
95
New England Biolabs nebnext sample purification beads
A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C <t>.</t> <t>Recombination</t> polymerase chain reactions were run on genomic <t>DNA</t> from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.
Nebnext Sample Purification Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/magic+pcr+preps+dna+purification+system/NEBNext+Ultra+II+FS+DNA+PCR-Free+Library+Prep+with+Sample+Purification+Beads/ppr0779265-78-54-54
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Image Search Results


A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C . Recombination polymerase chain reactions were run on genomic DNA from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.

Journal: bioRxiv

Article Title: Deletion of Murine Endothelial Bag3 Alters the Cellular Proteome Among Organs of Origin

doi: 10.1101/2025.09.24.678094

Figure Lengend Snippet: A. Cdh5(PAC)-CreERT2; Bag3 f/f mice were bred to generate homozygous deletion littermates. B. All mice received I.P. injections of either tamoxifen (KO; n=18) or vehicle (WT; n=18) at eight weeks of age. C. Fluorescence-activated cell sorting (FACS) was performed to isolate cells from the limb skeletal muscles of WT and KO mice, including unsorted cell populations and an endothelial cell specific fraction. C . Recombination polymerase chain reactions were run on genomic DNA from FACS-endothelial cells verifying exons 2-3 of the Bag3 gene were deleted (∼246bp) in the endothelial cells of KO mice with no deletion/recombination in the WT mice (∼468bp). D . mRNA expression was verified via qPCR. Values are presented as corrected for 18s, normalized to WT values (Fold Change – FC), means + SE.

Article Snippet: Tamoxifen induced recombination was confirmed through direct PCR analysis of genomic DNA using a DNA Isolation Kit (QuantaBio ExtractaBio DNA Prep, #95091-025).

Techniques: Fluorescence, FACS, Muscles, Expressing